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A. Confocal 3D scans showing the monocytes on top of the epithelial barrier, at 72 hours cocultures of ALI epithelia with monocytes. B. Lateral view of the 3D scan showing the monocytes on top of epithelial cells, not passing through the tight junctions. C. Dot-plot illustrating that monocytes can be isolated from cocultures with ALI epithelium by expression of <t>CD45</t> and CD14 D-E. Upon contact with epithelium blood monocytes increased MERTK expression as illustrated MFI of MERTK (D) and by % of MERTK positive cells (E). Each dot represents monocytes from 3 different patients on 3 different ALI epithelial systems (N=3). Statistical analysis was performed using One-Way Anova with Tukey’s multiple comparisons test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Data is presented as bar plot +/- SD of mean. F. SARS-Cov-2 Infected bronchial cells produced MCSF and CCL2, but no GM- CSF, at 72 hours. Each group is represented by multiple transwell systems that were used as control or infected with SARS-CoV-2, Mock N= 6, SARS-CoV-2 infected epithelia N= 9. Data is presented as bar plot with +/- SD of mean. Statistical comparison was performed using unpaired T test between different conditions. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 G. The IFN-β stimulated epithelium did not affect GM-CSF production. IFN-β pre-treated cocultures had lower levels of GM-CSF compared to controls at 72 hours (left graph). IFN-β stimulation resulted in higher M-CSF production from the epithelium at 24 hours. At 72 hour, the cocultures pre-treated with IFN-β also had higher M-CSF levels than the controls (right). Each group is represented by multiple transwell systems that were used as control or stimulated with 10 ng/mL IFN-β (24 hours stimulated cultures N=4, 72 hours cocultures N= 3). Data is presented as bar plot with mean +/- SD. Statistical comparison was performed using unpaired T test between different conditions. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001
Anti Cd45, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
anti cd45 - by Bioz Stars, 2026-02
93/100 stars
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A. Confocal 3D scans showing the monocytes on top of the epithelial barrier, at 72 hours cocultures of ALI epithelia with monocytes. B. Lateral view of the 3D scan showing the monocytes on top of epithelial cells, not passing through the tight junctions. C. Dot-plot illustrating that monocytes can be isolated from cocultures with ALI epithelium by expression of CD45 and CD14 D-E. Upon contact with epithelium blood monocytes increased MERTK expression as illustrated MFI of MERTK (D) and by % of MERTK positive cells (E). Each dot represents monocytes from 3 different patients on 3 different ALI epithelial systems (N=3). Statistical analysis was performed using One-Way Anova with Tukey’s multiple comparisons test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Data is presented as bar plot +/- SD of mean. F. SARS-Cov-2 Infected bronchial cells produced MCSF and CCL2, but no GM- CSF, at 72 hours. Each group is represented by multiple transwell systems that were used as control or infected with SARS-CoV-2, Mock N= 6, SARS-CoV-2 infected epithelia N= 9. Data is presented as bar plot with +/- SD of mean. Statistical comparison was performed using unpaired T test between different conditions. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 G. The IFN-β stimulated epithelium did not affect GM-CSF production. IFN-β pre-treated cocultures had lower levels of GM-CSF compared to controls at 72 hours (left graph). IFN-β stimulation resulted in higher M-CSF production from the epithelium at 24 hours. At 72 hour, the cocultures pre-treated with IFN-β also had higher M-CSF levels than the controls (right). Each group is represented by multiple transwell systems that were used as control or stimulated with 10 ng/mL IFN-β (24 hours stimulated cultures N=4, 72 hours cocultures N= 3). Data is presented as bar plot with mean +/- SD. Statistical comparison was performed using unpaired T test between different conditions. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001

Journal: bioRxiv

Article Title: PROS1 released by human lung basal cells upon SARS-CoV-2 infection facilitates epithelial cell repair and limits inflammation

doi: 10.1101/2024.09.11.612489

Figure Lengend Snippet: A. Confocal 3D scans showing the monocytes on top of the epithelial barrier, at 72 hours cocultures of ALI epithelia with monocytes. B. Lateral view of the 3D scan showing the monocytes on top of epithelial cells, not passing through the tight junctions. C. Dot-plot illustrating that monocytes can be isolated from cocultures with ALI epithelium by expression of CD45 and CD14 D-E. Upon contact with epithelium blood monocytes increased MERTK expression as illustrated MFI of MERTK (D) and by % of MERTK positive cells (E). Each dot represents monocytes from 3 different patients on 3 different ALI epithelial systems (N=3). Statistical analysis was performed using One-Way Anova with Tukey’s multiple comparisons test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Data is presented as bar plot +/- SD of mean. F. SARS-Cov-2 Infected bronchial cells produced MCSF and CCL2, but no GM- CSF, at 72 hours. Each group is represented by multiple transwell systems that were used as control or infected with SARS-CoV-2, Mock N= 6, SARS-CoV-2 infected epithelia N= 9. Data is presented as bar plot with +/- SD of mean. Statistical comparison was performed using unpaired T test between different conditions. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 G. The IFN-β stimulated epithelium did not affect GM-CSF production. IFN-β pre-treated cocultures had lower levels of GM-CSF compared to controls at 72 hours (left graph). IFN-β stimulation resulted in higher M-CSF production from the epithelium at 24 hours. At 72 hour, the cocultures pre-treated with IFN-β also had higher M-CSF levels than the controls (right). Each group is represented by multiple transwell systems that were used as control or stimulated with 10 ng/mL IFN-β (24 hours stimulated cultures N=4, 72 hours cocultures N= 3). Data is presented as bar plot with mean +/- SD. Statistical comparison was performed using unpaired T test between different conditions. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001

Article Snippet: For isotype of anti-CD45, mouse IgG2b was used (Novus-Bio, NBP1-43317), whereas for anti-TJP1, rabbit IgG (Vector Laboratories, I-1000) was used.

Techniques: Isolation, Expressing, Infection, Produced, Control, Comparison